Clustal W - Multiple Sequence Alignment - Use this to check multiple sequences (DNA or protein).Try using the Ensembl Gene ID input for actin beta (ENSG00000075624). protein_coding, lincRNA), accession IDs in different databases, etc. bioDBnet: db2db - Database to Database Conversions - Really useful for RNA-seq and other large-scale experiments! If you only have a list of gene names or IDs, use db2db to generate a list of gene name synonyms, gene descriptions, biotypes (e.g.Genome browsers, expression mappers (eFP browsers), etc. The Bio-Analytic Resource (BAR) for Plant Biology - Useful tools specifically geared for plant biologists.The results are from published data, so unstudied proteins will mistakenly look like they don't interact with anything. Input a list of proteins and see if they are known to interact. - Make protein signaling and interaction networks.Primer-BLAST - Use this tool to design specific primers for a gene of interest for qPCR or regular PCR.My favorite free software for viewing annotated files is SnapGene Viewer.
SnapGene, SeqBuilder, Benchling, ApE) and the result will be the annotation showing up right next to the DNA sequence. That GenBank file can be opened with any sequence software that supports annotations (e.g. For example, look up the cDNA sequence of actin beta, click on "Send:" in the top right corner, choose "Complete Record", choose destination "File", and download format "Genbank" or "Genbank (full)". GenBank - NIH Genetic Sequence Database- Use this to find annotated sequence data for genes of interest.For example: the ID for the gene encoding actin beta is ENSG00000075624. The Ensembl Gene IDs are useful for RNA-seq analysis. Ensembl- Go here for gene, genome sequence, and splicing isoform information.The scientific community with a comprehensive, high-quality andįreely accessible resource of protein sequence and functional Find information on functional and structural domains, calculate pI, calculate molecular weight, find homologs, get expression information and protein ontology notes. - Go here to find information on specific proteins.
0 kommentar(er)
